The Spectrophotometric Sulfo-Phospho-Vanillin Assessment of Total Lipids in Human Meibomian Gland Secretions
نویسندگان
چکیده
منابع مشابه
Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction.
to the compounds tested, for at least an hour under the described conditions, but biologically reliable and physiologically representative results probably can be obtained for a much longer time when the reasons for tubular damage have been identified and corrected. Aside from the fact that different metabolites were studied, our experimental arrangement differed from that used in studies of th...
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OBJECTIVE To determine whether androgen receptors affect the fatty acid profiles of neutral and polar lipids in human meibomian gland secretions. METHODS Meibomian gland secretion samples were obtained from both eyes of (1) women with complete androgen insensitivity syndrome, a condition characterized by dysfunctional androgen receptors, and (2) age-matched female and male controls. Samples w...
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Simple surgery gave whole meibomian glands of steer yielding approximately 47 mg of lipid per pair of lids. This lipid gave a thin-layer chromatography pattern similar to that of steer or human excreta. Column chromatography of pooled steer gland lipids and pooled human lipid (excreta) gave 0.06% and 7.54% hydrocarbons, respectively, containing no squalene. Excluding human hydrocarbons (as exog...
متن کاملThe photometric determination of gangliosides with the sulfo-phospho-vanillin reaction.
A simple, quantitative method is described for the photometric determination of gangliosides. The procedure is based on the sulfo-phospho-vanillin reaction, and does not require prior hydrolysis. It has been shown that the reaction is probably due to oxidation by sulfuric acid of the sphingosine moiety which results in the formation of aldehydes or ketones or both which then react with the phos...
متن کاملInfluence of aging on the polar and neutral lipid profiles in human meibomian gland secretions.
OBJECTIVE To determine whether aging is associated with significant alterations in the polar and neutral lipid profiles in human meibomian gland secretions. METHODS Meibomian gland secretions were collected from both eyes of younger and older men and women. Samples were processed for high-performance liquid chromatography or mass spectrometry and for the analysis of associated spectra of frag...
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ژورنال
عنوان ژورنال: Lipids
سال: 2013
ISSN: 0024-4201,1558-9307
DOI: 10.1007/s11745-013-3755-9